Brightfield Microscopy, pages 3-8.

 

Carefully read this exercise in preparation for using the microscope.  If time permits you may practice the procedures on pages 7-8, using a prepared slide.

 

Then answer the questions in the Laboratory Report on pages 9-11.  You should obtain a folder to contain all Lab Reports that are assigned on this LAB AID page.  This should be the first Lab report in the folder.  The folder should be the kind that holds pages, but do not use a ring binder.  Your Lab Reports will be handed in for grading twice during the semester.  Check the schedule for dates.

 

Protozoans, Algae, and Cyanobacteria, pages 33-41

Microscopic Invertebrates

 

This exercise will give you practice in the use of the microscope and will acquaint you with several groups of microscopic organisms.

 

Make a wet-mount of pond water with algae.  You will find various protozoa and microscopic invertebrates among the algae.

 

Find as many microorganisms as you can and record them on the Laboratory Report on page 43.  We will not attempt to identify all organisms.  If possible determine if they are green algae, diatoms, protozoa, or invertebrates which might include worms and insect-like creatures.

 

 

 

 

 

 

For this exercise you should collect some pond water from various sources and bring it to class.  Continue searching for Eucaryotic Microbes.  Add your results to the Lab Report on pages 43-45.

The Fungi

Pages 53-58

Study various living cultures and prepared slides of yeasts and molds.

Make drawings on the Lab Report on pages 59-60 and include these pages in your Lab Reports.

 

 

 

 

 

 

 

Ubiquity of Bacteria, pages 47-48

 

You will work in groups to prepare Nutrient Agar and pour it into Petri Plates, one plate per student.  Then expose the plates according to Table 6.1 on page 48.

 

Incubate the plates until the next period and then record results on pages 49-50.

Negative Staining, pages 93-94

 

Follow procedures in your Lab Book.

Use bacteria from cultures for staining and also bacteria from between your teeth.  Follow the procedural diagram on page 93.

 

Record data on Lab Report, page 97.

 

 

Follow this procedure on the same slide you started with Smear Preparation, Oral Bacteria from your teeth.  Make a drawing on page 97, part 1.

Gram Staining, pages 101-104

 

Follow the procedure pictured on page 102.  Start with a heat fixed smear.  Prepare at least two slides.  1.  Oral Bacteria  2.  Bacteria from your culture.  Include drawings on page 111.  Learn this procedure for the Unknown Exam.

Spore Staining, pages 105-106

 

Use only the Schaeffer-Fulton Method pictured on page 106.  Start with a heat fixed slide from your culture.  Spores should be green.  If no spores present, red.  Include drawings on page 111.  Learn this procedure for the Unknown Exam.

Acid Fast Staining, pages 109-110

 

Start with a heat fixed slide from a culture provided.  Follow procedure on page 110.  Mycobacterium should stain red.  Other bacteria should stain blue.  Draw your results on page 112.  Learn this procedure for the Unknown Exam.

 

Go to the page on The Unknown Exam and read how to study for it.

Will be due October 17, 2006.

 

Pages should be bound into a folder.

No loose pages.  No ring binders.

Only Lab Reports and Film Report, nothing else.

Your name should be on the outside of the folder.

All Labs listed on this page should have a report.

Come to class with the folder ready to hand in on October 17.